Phytochemical Analysis in Callus Cultures of Podophyllum hexandrum

Abstract

The endangered medicinal plant Podophyllum hexandrum is a member of the Berberidaceae family. The rhizome contains a lot of P. hexandrum's lignans, which have anticancer properties. The most powerful cytotoxic substance that occurs naturally is podophyllotoxin. The anticancer medications etoposide and teniposide are made from it as a basic material. The formation of microtubules is prevented by podophyllotoxin. From the callus of Podophyllum hexandrum that were aseptically cultivated, callus cultures were created. A fully defined MS medium supplemented with several PGRs was efficient for both the beginning and continuation of callus tissue formation. Plant growth regulators significantly impacted the relative proportion of callus. Elicitors were used for the enhancement of biomass and podophyllotoxin production.