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Title: 2D Map of Proteins from Human Renal Stone Matrix and Evaluation of their Effect on Oxalate Induced Renal Tubular Epithelial Cell Injury
Authors: Aggarwal, K.P.
Tandon, S.
Singh, S.K.
Tandon, C.
Keywords: Urolithiasis
Calcium Oxalate
Madin Darby Canine Kidney Cells
Issue Date: 2013
Publisher: Jaypee University of Information Technology, Solan, H.P.
Abstract: Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate injured renal epithelial cells accompanied by a 2D map of these proteins. Materials and Methods: Proteins were isolated from the matrix of kidney stones containing CaOx as the major constituent using EGTA as a demineralizing agent. The effect of more than 3kDa proteins from matrix of human renal (calcium oxalate) CaOx stones was investigated on oxalate induced cell injury of MDCK renal tubular epithelial cells. A 2D map of >3kDa proteins was also generated followed by protein identification using MALDI-TOF MS.
Description: International Brazilian Journal of Urology, Vol. 39 (1): 128-136, January - February, 2013 doi: 10.1590/S1677-5538.IBJU.2013.01.16
ISSN: 1677-5538
Appears in Collections:Journal Articles

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