Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSharma, Sanchita-
dc.contributor.authorSood, Hemant [Guided by]-
dc.description.abstractPicrorhiza kurroa is a medicinally important perennial herb found at an altitude of 3000-5000 m commonly known as “Kutki” or “Kutaki” from Scrophulariaceae plant family located in the Himalayan region. Plant is now listed as endangered species. It has many valuable compounds which offer many uses of Picrorhiza kurroa such as hepatoprotective, antioxidant, anti-inflammatory, antiallergic, antiasthmatic, anti-oxidant, anticancerous, anti-bacterial, anti-anaphylactic, anti-malarial, cardioprotective and immune-modulating and potential for treatment of vitiligo. The two main iridoid compounds present in Picrorhiza kurroa are Picroside-I and Picroside-II which are used in many herbal drugs commercially available in different combinations. P. kurroa plant chemotypes have varied amount of P-I when grown in field conditions. Two chemotypes of high Picroside-I content PKS-1 and PKS-21 and two of low Picroside-I content PKS-4 and PKS-5 of P. kurroa were selected. The Micropropagation in multiple shoot induction medium showed 21.33 as highest no of shoots, 5.98 cm as highest shoot length and 35.60 g highest number of shoot biomass in PKS-4 followed by PKS-1, PKS-5 and least in PKS-21. The highest P-I content 0.1961mg/g was observed in PKS-21 followed by PKS-1, PKS-5 and least in PKS-4. The gene expression analysis showed expression levels of G10H, PAL, ISPE, HMGR, DXPS, GS, PMK in accordance with levels of P-I which was found to be highest in PKS-21 which proved the association of these genes to P-I synthesis. So the interventions in these genes can be done to increase the P-I content in P. kurroa.en_US
dc.publisherJaypee University of Information Technology, Solan, H.P.en_US
dc.subjectPicrorhiza kurroaen_US
dc.subjectDeoxyribo nucleic aciden_US
dc.subjectMolecular characterizationen_US
dc.titleMicropropagation and Molecular Characterization of Picrorhiza Kurroa Chemotypes for Picroside-i Productionen_US
dc.typeProject Reporten_US
Appears in Collections:Dissertations (M.Tech.)

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.