Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/7234
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSrivastava, Pulkit Anupam-
dc.contributor.authorYennamalli, Ragothaman M. [Guided by]-
dc.date.accessioned2022-09-30T12:38:29Z-
dc.date.available2022-09-30T12:38:29Z-
dc.date.issued2019-
dc.identifier.urihttp://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/7234-
dc.description.abstractLytic polysaccharide monooxygenases (LPMO), a family of copper-dependent oxidative enzymes, boost the degradation of crystalline polysaccharides, such as cellulose and chitin, by breaking an internal glycosidic bond thereby exposing the polymer for further degradation. Recently, the sequence diversity of LPMOs has increased significantly, with newer sequences identified in organisms across the tree of life. Accurate functional assignment of yet unknown sequences into LPMOs family is an important step towards production of enzymatic mixture adept at efficiently degrading recalcitrant polysaccharides. While, multiple experimental methods are used for accurate identification of LPMOs, a computational method that can accurately classify sequences into LPMOs is needed to match the sequences generated. Thus, to screen potential LPMOs, we developed a machine learning based tool that employs two different approaches to functionally classify a given protein sequence(s) as belonging to LPMO family or not. As proof of concept, we worked on classifying sequences belonging to either AA9 or AA10 family of LPMO. The first approach uses traditional neural network based prediction after calculating sequence features. The second approach uses bi-directional long short-term memory (LSTM) units, a type of recurrent neural network, which extracts important features directly from sequence and utilizes an internal state, i.e., memory, to process input data. The optimized model trained from both the approaches was cross validated on a validation set to test the precision and recall. Specifically, feature-based traditional neural network approach was able to correctly discriminate AA9 LPMO sequences from non-AA9 LPMOs with a recall of 96.4%, precision of 100% and AA10 LPMO sequences from non-AA10 LPMOs with a recall of 86.9%, precision of 100%. On the other hand, LSTM had a recall of 93.4%, precision of 90.7% on AA9 dataset and recall of 91.7%, precision of 89.6% on AA10 dataset. Further, we validated our method with an independent benchmark set of LPMO sequences, where we observed significant precision and recall compared to dbCAN2, an existing HMM-profile based CAZyme predicting tool. The working code can be freely found at: https://github.com/PulkiD/PreDSLpmo.en_US
dc.language.isoenen_US
dc.publisherJaypee University of Information Technology, Solan, H.P.en_US
dc.subjectLytic polysaccharide monooxygenasesen_US
dc.subjectDeep neural networken_US
dc.subjectLong short-term memoryen_US
dc.subjectProteomeen_US
dc.titleMachine Learning Based Approach for Functional Annotation of Lytic Polysaccharide Monooxygenasesen_US
dc.typeProject Reporten_US
Appears in Collections:B.Tech. Project Reports



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.