Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/8586
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dc.contributor.authorPal, Tarun-
dc.contributor.authorPadhan, Jibesh Kumar-
dc.contributor.authorKumar, Pawan-
dc.contributor.authorSood, Hemant-
dc.contributor.authorChauhan, Rajinder S.-
dc.date.accessioned2022-12-14T05:04:42Z-
dc.date.available2022-12-14T05:04:42Z-
dc.date.issued2018-
dc.identifier.urihttp://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/8586-
dc.description.abstractSwertia chirayita is a high-value medicinal herb exhibiting antidiabetic, hepatoprotective, anticancer, antiediematogenic and antipyretic properties. Scarcity of its plant material has necessitated in vitro production of therapeutic metabolites; however, their yields were low compared to field grown plants. Possible reasons for this could be differences in physiological and biochemical processes between plants grown in photoautotrophic versus photoheterotrophic modes of nutrition. Comparative transcriptomes of S. chirayita were generated to decipher the crucial molecular components associated with the secondary metabolites biosynthesis. Illumina HiSeq sequencing yielded 57,460 and 43,702 transcripts for green house grown (SCFG) and tissue cultured (SCTC) plants, respectively. Biological role analysis (GO and COG assignments) revealed major differences in SCFG and SCTC transcriptomes. KEGG orthology mapped 351 and 341 transcripts onto secondary metabolites biosynthesis pathways for SCFG and SCTC transcriptomes, respectively. Nineteen out of 30 genes from primary metabolism showed higher in silico expression (FPKM) in SCFG versus SCTC, possibly indicating their involvement in regulating the central carbon pool. In silico data were validated by RT-qPCR using a set of 16 genes, wherein 10 genes showed similar expression pattern across both the methods. Comparative transcriptomes identified differentially expressed transcription factors and ABC-type transporters putatively associated with secondary metabolism in S. chirayita. Additionally, functional classification was performed using NCBI Biosystems database. This study identified the molecular components implicated in differential modes of nutrition (photoautotrophic vs. photoheterotrophic) in relation to secondary metabolites production in S. chirayita.en_US
dc.language.isoenen_US
dc.publisherJaypee University of Information Technology, Solan, H.P.en_US
dc.subjectSwertia chirayitaen_US
dc.subjectSecondary metabolitesen_US
dc.subjectTranscriptomeen_US
dc.subjectNext generation sequencingen_US
dc.subjectPhotoautotrophicen_US
dc.subjectPhotoheterotrophicen_US
dc.titleComparative transcriptomics uncovers differences in photoautotrophic versus photoheterotrophic modes of nutrition in relation to secondary metabolites biosynthesis in Swertia chirayitaen_US
dc.typeArticleen_US
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