Please use this identifier to cite or link to this item: http://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9025
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dc.contributor.authorSharma, Arti-
dc.contributor.authorChauhan, Rajinder Singh-
dc.date.accessioned2023-01-09T05:30:30Z-
dc.date.available2023-01-09T05:30:30Z-
dc.date.issued2012-
dc.identifier.urihttp://ir.juit.ac.in:8080/jspui/jspui/handle/123456789/9025-
dc.description.abstractJatropha (Jatropha curcas) and castor bean (Ricinus communis) possess several taxonomic similarities, and their seeds contain a high proportion of oil (up to 40%) which has been used in various industrial products, including diesel oil. Thirty-two candidate genes responsible for fatty acid biosynthesis were identified in the castor bean genome sequence. Testing of 48 primer pairs from candidate gene regions, including 12 SSRs from castor bean on 54 genotypes of J. curcas, 65% amplified successfully on Jatropha out of which 20% showed polymorphisms. Jatropha genotypes, categorized for oil content, were used in association analysis of candidate gene regions with high oil content. One marker–trait association for the oil trait was identified. Stearoyl desaturase amplicon (700 bp) consisting of intron and exon (P00.00013) showed association with high oil content in Jatropha genotypes. Sequencing of the 1.3-kb amplicon, including the 700-bp fragment of stearoyl desaturase, which had shown association with the high oil content, revealed SNPs in the exonic region. The SNPs resulted in substitution of leucine with glutamine in the open reading frame of stearoyl desaturase of low oil content genotypes. The molecular marker is expected to be useful in marker-assisted breeding of high oil content genotypes in Jatropha.en_US
dc.language.isoenen_US
dc.publisherJaypee University of Information Technology, Solan, H.P.en_US
dc.subjectCastor beanen_US
dc.subjectComparative genomicsen_US
dc.subjectFatty acid biosynthesisen_US
dc.subjectJatrophaen_US
dc.titleIdentification and Association Analysis of Castor Bean Orthologous Candidate Gene-Based Markers for High Oil Content in Jatropha curcasen_US
dc.typeArticleen_US
Appears in Collections:Journal Articles



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